Chromatography

Chromatography-Mass Spectroscopy Analysis

Liquid chromatography is an abecedarian separation fashion in the life lores and related fields of chemistry. Unlike gas chromatography, which is infelicitous for non-volatile and thermally fragile motes, liquid chromatography can safely separate a veritably wide range of organic composites, from small-patch medicine metabolites to peptides and proteins. Traditional sensors for liquid chromatography include a refractive indicator, electrochemical, luminescence, and ultraviolet-visible (UV-Vis) sensors. Some of these induce two-dimensional data; that is, data representing signal strength as a function of time. Others, including luminescence and diode array UV-Vis sensors, induce three-dimensional data. Three-dimensional data include not only signal strength but spectral data for each point in time.

Mass spectrometers also induce three-dimensional data. In addition, to signal strength, they induce mass spectral data that can give precious information about the molecular weight, structure, identity, volume, and chastity of a sample. Mass spectral data add particularity that increases confidence in the results of both qualitative and quantitative analyses.