Chromatography as Separation Techniques

High-Performance Liquid Chromatography (HPLC) is the anon-destructive procedure for resolving a complex admixture into its individual fragments or composites. It's grounded on the discrimination migration of solutes with the detergents. Those solutes with a high affinity for the mobile phase will spend further time in this phase than the solutes that prefer the stationary phase. As the solute rises up through the stationary phase they separate. The process is called chromatographic development. The bit with lesser affinity to stationary sub caste peregrination slower and shorter distance while that with lower affinity peregrination briskly and longer.

 

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